Shin dihydrocoumarin yana da guba?

Dihydrocoumarin, ƙamshi, wanda ake amfani da shi a abinci, wanda kuma ake amfani da shi azaman madadin coumarin, ana amfani da shi azaman ɗanɗanon kwalliya; Man shafawa, kwakwa, ɗanɗanon kirfa; Hakanan ana amfani da shi azaman ɗanɗanon taba.

Shin dihydrocoumarin yana da guba?

Dihydrocoumarin ba shi da guba. Dihydrocoumarin wani samfuri ne na halitta da ake samu a cikin rhino mai launin rawaya na vanilla. Ana shirya shi ta hanyar hydrogenation na coumarin a gaban sinadarin nickel a zafin 160-200 ℃ kuma a ƙarƙashin matsin lamba. Hakanan ana iya amfani da shi azaman kayan da aka samar, a sanya shi a cikin ruwan alkaline don samar da o-hydroxyphenylpropionic acid, bushewa, da rufewa.

Yanayin Ajiya

A rufe kuma duhu, an adana shi a wuri mai sanyi da bushewa, sararin da ke cikin ganga yana da ƙanƙanta gwargwadon iko a ƙarƙashin izinin aminci, kuma an cika shi da kariyar nitrogen. A adana a cikin ma'ajiyar ajiya mai sanyi da iska. A guji wuta, ruwa. Ya kamata a adana shi daban da na'urar tace iska, kada a haɗa wurin ajiya. An sanye shi da nau'ikan kayan aikin kashe gobara da yawa.

Nazarin In Vitro

Gwajin enzymatic a cikin vitro, dihydrocoumarin ya haifar da hana SIRT1 mai dogaro da yawan maida hankali (IC50 na 208μM). An lura da raguwar ayyukan deacetylase na SIRT1 har ma a allurai na micromolar (85±5.8 da 73± 13.7% na aiki a 1.6μM da 8μM, bi da bi). An kuma hana microtubule SIRT2 deacetylase ta hanyar da ta dogara da kashi (IC50 na 295μM).

Bayan awanni 24 na fallasa, dihydrocoumarin (1-5mM) ya ƙara yawan guba a cikin layukan ƙwayoyin TK6 ta hanyar da ta dogara da kashi. Dihydrocoumarin (1-5mM) ya ƙara yawan apoptosis a cikin layukan ƙwayoyin TK6 ta hanyar da ta dogara da kashi a cikin awanni 6. Adadin dihydrocoumarin na 5mM ya ƙara yawan apoptosis a cikin awanni 6 a cikin layin ƙwayoyin TK6. Bayan lokacin fallasa na awanni 24, dihydrocoumarin (1-5mM) ya ƙara yawan acetylation na p53 lysine 373 da 382 ta hanyar da ta dogara da kashi a cikin layin ƙwayoyin TK6.